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Finally, the secondary-metabolites of selected APH were screened through literature search, Lipinski rule, ADMET, and ProTox-II. Among the tested extracts, I-9-HAE (α-amylase inhibition: IC50 831.84 µg/ml) and MN-HAE (α-glucosidase inhibition: IC50 558.64 µg/ml and antiglycation: IC50 883.74 µg/ml) have showed highest antihyperglycemic and antiglycation properties.

Furthermore, three APH with better antiradical potential was chosen for various in vitro and in silico method, for validating scientific antidiabetic propensities. A significant correlation was obtained between TPC-DPPH⁺ (r²: 0.8187****).

⁺: IC50 2.71 µg/ml), and MN-HAE (FRAP and TAC) exhibited highest antioxidant capacity.

⁺ values were higher compared to DPPH⁺ assay.

Therefore, present study aims to validate the sensitivity/capacity of different antioxidant assay, thereby assessing the antioxidant potential of 9-APH. Subsequently selecting an antioxidant assessment method is a challenging aspect, considering various in vitro assays working with diverse mechanism of action. Therefore, assessing antioxidant activity of various APH might unveil an antioxidant-rich formulation for management of DM and its associated complications. High glucose levels in DM are related to oxidative stress leading to its associated complications. In conclusion, this study provides a possible synthetic method for developing novel and nontoxic antioxidant agents which can be used in the food and cosmetics industry.Īntidiabetic polyherbal formulations (APH) are used in management of diabetes mellitus (DM). The cytotoxicity assay of L929 cells in vitro indicated that the derivatives had low cytotoxicity and good biocompatibility. Moreover, the study showed that the phenolic acid chitooligosaccharide salts had stronger antioxidant activity than phenolic-acid-acylated chitooligosaccharide. The antioxidant experiment results in vitro (including DPPH-radical scavenging activity, superoxide-radical scavenging activity, hydroxyl-radical scavenging ability, and reducing power) demonstrated that the derivatives exhibited significantly enhanced antioxidant activity compared to COS. The structures of the derivatives were characterized by FT-IR and 1H NMR spectra. Seven phenolic acids were selected to combine two kinds of COS derivatives: the phenolic acid chitooligosaccharide salt derivatives and the phenolic-acid-acylated chitooligosaccharide derivatives.

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glyptostroboides or its secondary metabolite taxoquinone as confirmed by its promising antioxidant and free radical scavenging activities.Ī series of phenolic acid chitooligosaccharide (COS) derivatives synthesized by two mild and green methods were illuminated in this paper. Conclusions: These findings justify the biological and traditional uses of M. Results: Taxoquinone showed significant and concentration-dependent antioxidant and free radical scavenging activities of DPPH, nitric oxide, superoxide and hydroxyl free radicals by 78.83%, 72.42%, 72.99% and 85.04%, as compared to standard compounds ascorbic acid (81.69%, 74.62%, 73% and 73.79%) and α-tocopherol/butylated hydroxyanisole (84.09%, 78.61%, 74.45% and 70.02%), respectively. Methods: An abietane type diterpenoid taxoquinone, isolated from ethyl acetate cone extract of Metasequoia glyptostroboides, was analyzed for its antioxidant efficacy as reducing power ability and its ability to scavenge free radicals such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide and hydroxyl radicals. Hence, this study was carried out to investigate the antioxidant and free radical scavenging efficacy of a biologically active diterpenoid compound taxoquinone isolated from Metasequoia glyptostroboides in various antioxidant models. Objective: In recent days, there is an upsurge of interest on finding effective phyto-constituents as new sources of natural antioxidants for using in food and pharmaceutical preparations.

Metasequoia free